Enzyme inhibitory and antioxidant activities and HPLC quantification of chlorogenic acid in Helichrysum stoechas (L.) Moench and H. stoechas subsp. barrelieri (Ten) Nyman

The inhibitory effects of ethanol (80%) and aqueous extracts Helichrysum stoechas (L.) Moench H. subsp. barrelieri (Ten) Nyman on acetylcholinesterase (AChE) butyrylcholinesterase (BChE), two sister enzymes associated with pathogenesis Alzheimer's disease (AD), as well elastase collagenase, linked to inflammation skin aging, were investigated. Simultaneously, antioxidant activity the was assessed through DPPH radical scavenging activity, ferric reducing power (FRAP), metal-chelating assays, since oxidative damage plays a critical role in both AD pathophysiology aging. Total phenol flavonoid contents spectrophotometrically determined. highest AChE (44.60 ± 4.4% at 2000 µg/mL) found extract collected from Hatay, uppermost BChE same concentration Izmir (80.24 2.63%, IC50: 38.52 1.41 µg/mL). Both them inhibited concentration-dependent manner. Nevertheless, none plants collagenase. Although ethanolic had significant FRAP they demonstrated inadequate assay. Chlorogenic acid quantified using HPLC. mentioned having strong cholinesterase (ChE) inhibition also chlorogenic content. (Hatay sample) (Izmir seem contain promising ChE inhibitors, which deserve further investigation.